论文标题
黑暗适应异常的固有信号光学图导致杆变性
Intrinsic signal optoretinography of dark adaptation abnormality due to rod degeneration
论文作者
论文摘要
显着性：多种眼科疾病，例如与年龄相关的黄斑变性，糖尿病性视网膜病和色素性视网膜炎会引起感光受体功能障碍。已知杆感光体比锥形光感受器更脆弱。因此，对杆感光体的功能评估对于早期检测眼病很重要。目的：这项研究是为了证明使用固有的光信号（IOS）摄影（ORG）的可行性，以实现由于Rod感光受体变性而导致的黑暗适应性（DA）异常。方法：用于野生型（WT）和视网膜变性10（RD10）小鼠的iOS ORG使用功能性光学相干断层扫描（OCT）。本研究使用了六个WT C57BL/6J和八个RD10 B6.CXB1-PDE6BRD10/J小鼠在第14天（P14）进行此研究。对应于光到黑暗转变的视网膜厚度和亮度变化的动态OCT分析结果：在光和黑暗条件下对视网膜的比较测量显示出视网膜外的iOS发生了显着变化。与光适应（LA）相比，在DA期间，DA期间内部段椭圆形区（EZ）的OCT亮度降低了，外部极限膜（ELM）和视网膜色素上皮（RPE）之间的厚度减小。与WT相比，观察到相对EZ强度的变化在RD10中降低。在DA期间，ELM和RPE之间的不足条带的相对强度也显示出RD10视网膜的显着降低。结论：与p14的WT相比，在RD10中观察到了DA-OIS异常。 DA-OIS动力学的组织测量有望获得有价值的信息，以评估光感受器功能。
Significance: Multiple eye diseases such as age-related macular degeneration, diabetic retinopathy, and retinitis pigmentosa can cause photoreceptor dysfunction. Rod photoreceptors are known to be more vulnerable than cone photoreceptors. Therefore, functional assessment of rod photoreceptors is important for early detection of eye diseases. Aim: This study is to demonstrate the feasibility of using intrinsic optical signal (IOS) optoretinography (ORG) for objective detection of dark adaptation (DA) abnormality due to rod photoreceptor degeneration. Approach: Functional optical coherence tomography (OCT) was employed for IOS ORG of wild-type (WT) and retinal degeneration 10 (rd10) mice. Six WT C57BL/6J and eight rd10 B6.CXB1-Pde6brd10/J mice at postnatal day 14 (P14) were used for this study. Dynamic OCT analysis of retinal thickness and brightness changes, corresponding to light to dark transition, was implemented Results: Comparative measurement of the retina under light and dark conditions revealed significant IOS changes within the outer retina. The thickness between external limiting membrane (ELM) and retinal pigment epithelium (RPE) reduced, and the OCT brightness of inner segment ellipsoid zone (EZ) decreased during DA, compared to light adaptation (LA). Relative EZ intensity change was observed to decrease larger in rd10, compared to WT. The relative intensity of the hyporeflective band between ELM and RPE also showed significant decrease in rd10 retinas during DA. Conclusions: DA-IOS abnormalities were observed in rd10, compared to WT at P14. The ORG measurement of DA-IOS kinetics promises valuable information for noninvasive assessment of photoreceptor function.
