论文标题
扩散引起的各向异性癌症侵袭：一种基于肿瘤球体的新型实验方法
Diffusion-induced anisotropic cancer invasion: a novel experimental method based on tumour spheroids
论文作者
论文摘要
肿瘤侵袭受微环境的强烈影响，除其他参数外，化学刺激起着重要作用。这里介绍了3D胶原凝胶中细胞球体形态学的实时成像在体外进行定量研究的创新方法。通过使用趋化腔室在球体上施加营养（葡萄糖）的受控梯度进行测定，模仿血管接近的趋化刺激。将不同的肿瘤细胞系（PANC-1和HT-1080）与非肿瘤的细胞系进行比较（NIH/3T3）。通过配备孵化系统并通过图像分析技术量化的延时工作站观察形态响应。对入侵现象的描述是基于基于运输现象概念的工程方法。正如预期的那样，NIH/3T3球体的特征是细胞侵入周围组织的趋势有限，这与PANC-1和HT-1080不同，对梯度的反应相对较强。
Tumour invasion is strongly influenced by microenvironment and, among other parameters, chemical stimuli play an important role. An innovative methodology for the quantitative investigation of chemotaxis in vitro by live imaging of morphology of cell spheroids, in 3D collagen gel, is presented here. The assay was performed by using a chemotactic chamber to impose a controlled gradients of nutrients (glucose) on spheroids, mimicking the chemotactic stimuli naturally occurring in the proximity of blood vessels. Different tumoral cell lines (PANC-1 and HT-1080) are compared to non-tumoral ones (NIH/3T3). Morphology response is observed by means a Time-lapse workstation equipped with an incubating system and quantified by image analysis techniques. Description of invasion phenomena was based on an engineering approach, based on transport phenomena concepts. As expected, NIH/3T3 spheroids are characterized by a limited tendency of cells to invade the surrounding tissue, unlike PANC-1 and HT-1080 that show relatively stronger response to gradients.
