论文标题
在非线性显微镜中应用的广义光谱唯一时间域的ptychographic阶段重建
Generalized spectral phase-only time-domain ptychographic phase reconstruction applied in nonlinear microscopy
论文作者
论文摘要
在过去的几十年中，非线性显微镜已经发展成为生物科学中成像和光谱应用的强大工具。在这项研究中，实施了一种新型的光谱相控制技术I $^2 $ PIE，以压缩全非正常分散（ANDI）光子晶体（PCF）中产生的宽带超脑脉冲。据我们所知，这项基于时间域PTYChography的技术首次在非线性显微镜应用中证明了这项技术。该技术在生物样品中的第二次谐波生成和两光兴奋荧光显微镜中的第一个现实应用。我们进一步表明，与非线性显微镜中使用的常规压缩技术相比，在实施中，I $^2 $ PIE可以改善生成图像中的对比度和信噪比。
Nonlinear microscopy has evolved over the last few decades to become a powerful tool for imaging and spectroscopic applications in biological sciences. In this study, I$^2$PIE, a novel spectral phase control technique, was implemented in order to compress broad-bandwidth supercontinuum light pulses generated in an all-normal-dispersion (ANDi) photonic crystal fiber (PCF). The technique, based on time-domain ptychography, is demonstrated here in a nonlinear microscopy application for the first time, to the best of our knowledge. The first real-world application of this technique for second-harmonic generation and two-photon excitation fluorescence microscopies in biological samples is presented. We further show that in our implementation, I$^2$PIE leads to improved contrast and signal-to-noise ratios in the generated images, compared to conventional compression techniques used in nonlinear microscopy.
