论文标题
分析健康和疾病中的核糖体重塑
Analyzing ribosome remodeling in health and disease
论文作者
论文摘要
调节在很大程度上没有探索，部分原因是方法论上的局限性。确实，我们回顾了证据，表明常见方法（例如转录组学）是不足的，因为编码核糖体蛋白（RP）的mRNA的可变性不一定与RP变异性相对应。因此，核糖体的蛋白质重塑应通过允许直接定量RP的方法（理想情况下是分离的核糖体）进行研究。我们回顾了这样的方法，重点是质谱和强调特定方法的偏见和控制这些偏见的方法。我们认为，使用多种互补方法可以帮助减少解释可再现的系统偏见作为核糖体重塑的证据的危险。
regulation largely unexplored, in part due to methodological limitations. Indeed, we review evidence demonstrating that commonly used methods, such as transcriptomics, are inadequate because the variability in mRNAs coding for ribosomal proteins (RP) does not necessarily correspond to RP variability. Thus protein remodeling of ribosomes should be investigated by methods that allow direct quantification of RPs, ideally of isolated ribosomes. We review such methods, focusing on mass spectrometry and emphasizing method-specific biases and approaches to control these biases. We argue that using multiple complementary methods can help reduce the danger of interpreting reproducible systematic biases as evidence for ribosome remodeling.
