论文标题
小分子特性对抗体反应的影响
Influence of Small Molecule Property on Antibody Response
论文作者
论文摘要
对于针对滥用滥用药物的疫苗，对毒素的解毒剂和化合物免疫测定法的疫苗，具有高滴度和对小分子亲和力的抗体至关重要。然而，关于小分子的特性如何影响以及哪种化学描述符表明抗体反应的程度，知之甚少。基于我们先前的研究，我们设计和合成了两组称为触觉的小分子，具有多种疏水性，以研究小分子的性质与滴度和亲和力的抗体反应之间的关系。我们发现抗体反应的大小与分子疏水性和相关化学描述符的程度呈正相关。这项研究提供了对小分子本身的免疫学特征和有用的线索，以产生针对小分子的高质量抗体。
Antibodies with high titer and affinity to small molecule are critical in the field for the development of vaccines against drugs of abuse, antidotes to toxins and immunoassays for compounds. However, little is known regarding how properties of small molecule influence and which chemical descriptor could indicate the degree of the antibody response. Based on our previous study, we designed and synthesized two groups of small molecules, called haptens, with varied hydrophobicities to investigate the relationship between properties of small molecules and antibody response in term of titer and affinity. We found that the magnitude of the antibody response is positively correlated with the degree of molecular hydrophobicity and related chemical descriptors. This study provides insight into the immunological characteristics of small molecules themselves and useful clues to produce high quality antibodies against small molecules.
