论文标题
通过相关的原位空间和热分析，在冷冻保存过程中揭示了细胞的本地环境
Unveiling cells' local environment during cryopreservation by correlative in situ spatial and thermal analyses
论文作者
论文摘要
冷冻保存是延长活细胞和组织寿命的唯一完全建立的程序，这是从基本生物学到临床实践的活动的关键。尽管它的盛行和影响，但冷冻保存的主要方面，例如冻结过程中细胞的物理化学环境，仍然难以捉摸。在这里，我们通过将原位显微镜定向冻结耦合，以与冰冻中相图在冷冻过程中可视化细胞及其周围环境，以解决这个问题。我们在冷冻保存中提取冰冻培养基空间分布，提供了一种在冷冻过程中任何一点描述细胞附近的工具。我们表明，随着时间的流逝，两个主要事件定义了细胞的本地环境：与移动冰面的相互作用以及玻璃体移动前部 - 我们在此处引入的术语。我们的相关策略可以应用于临床研究和实践中相关的细胞，并帮助基于局部物理化学提示设计新的冷冻保护介质。
Cryopreservation is the only fully established procedure to extend the lifespan of living cells and tissues, a key to activities spanning from fundamental biology to clinical practice. Despite its prevalence and impact, central aspects of cryopreservation, such as the cell's physico-chemical environment during freezing, remain elusive. Here we address that question by coupling in situ microscopic directional freezing to visualize cells and their surroundings during freezing with the freezing medium phase diagram. We extract the freezing medium spatial distribution in cryopreservation, providing a tool to describe the cell vicinity at any point during freezing. We show that two major events define the cells' local environment over time: the interaction with the moving ice front and with the vitreous moving front - a term we introduce here. Our correlative strategy may be applied to cells relevant in clinical research and practice, and help designing new cryoprotective media based on local physico-chemical cues.
