论文标题

放松对数据获取和位置检测的限制,用于光学镊子中的陷阱刚度校准

Relaxing constraints on data acquisition and position detection for trap stiffness calibration in optical tweezers

论文作者

Melo, Bruno, Almeida, Felipe, Temporão, Guilherme, Guerreiro, Thiago

论文摘要

光学镊子在各个领域找到应用,从生物学到物理学。使用被困颗粒进行定量测量所需的基本步骤之一是镊子弹簧常数的校准。这可以通过功率光谱密度分析来完成,从粒子位置的正向散射检测。在这项工作中,我们提出并实验测试了此类测量程序的简化,旨在减少记录数据的后处理,并处理具有频率依赖性电子噪声的采集设备。在简化镊子设置的同一条线中,我们还提出了一个可以替代标准位置敏感探测器的刀具检测方案。

Optical tweezers find applications in various fields, ranging from biology to physics. One of the fundamental steps necessary to perform quantitative measurements using trapped particles is the calibration of the tweezer's spring constant. This can be done through power spectral density analysis, from forward scattering detection of the particle's position. In this work, we propose and experimentally test simplifications to such measurement procedure, aimed at reducing post-processing of recorded data and dealing with acquisition devices that have frequency-dependent electronic noise. In the same line of simplifying the tweezer setup, we also present a knife-edge detection scheme that can substitute standard position-sensitive detectors.

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