论文标题
蛋白质结晶期间使用限制抑制对流强度
Suppressing convection strength using confinement during protein crystallization
论文作者
论文摘要
蛋白质结晶过程中的流体对流在确定晶体生长速率和晶体质量方面起着重要作用。以降低的流量生长的晶体可提供更好的质量晶体。因此，调整流动强度在晶体生长过程中至关重要。在这项工作中，我们展示了一种新方法，可以在蛋白质结晶的蒸气扩散方法中使用限制效应抑制流量强度，其中晶体在蒸发的液滴内生长。流动研究是在四个不同的限制条件下进行的，以研究限制的影响。液滴内部的流量是由蒸发引起的自然对流引起的，该对流是通过Micro-PIV方法测量的。同样在生长晶体周围产生的浓度梯度也会在晶体生长阶段引起蛋白质晶体周围的浮力驱动流动。液滴的蒸发速率和液滴内部的流动强度通过增加的限制而抑制。因此，可以通过调整限制来调整流量强度，这是调整流动强度的非常简单的方法。
Fluid convection during protein crystallization plays a significant role in determining crystal growth rate and crystal quality. Crystals grown in reduced flow strength gives better quality crystal. Hence, tuning the flow strength is very essential in the crystal growth process. In this work, we have demonstrated a new method to suppress the flow strength using confinement effect during vapor diffusion method of protein crystallization where the crystal is grown inside an evaporating droplet. Flow study is carried out at four different confinement conditions to study the effect of confinement. Flow inside the droplet is caused by evaporation induced natural convection which is measured by micro-PIV method. Also concentration gradient generated around the growing crystal also induces buoyancy driven flow around the protein crystal during the crystal growth phase. The evaporation rate from the droplet and the flow strength inside the droplet get suppressed by increasing confinement. Hence, the flow strength can be tuned by adjusting the confinement which is a very simple method to tune the flow strength.
