论文标题
简单的翻译后昼夜节律时钟模型
Simple post-translational circadian clock models from selective sequestration
论文作者
论文摘要
在没有负面反馈转录抑制的情况下，来自不同生物体的许多细胞类型的反馈转录抑制作用，可能会继续运作后，有些翻译后循环振荡器可能会继续运作。除了来自蓝细菌的KaiaBC系统外，目前尚不清楚在不同细胞类型中创建体外（“测试管”）昼夜节律所需的分子成分和相互作用。受KAIABC系统的启发，我提供了“原理证明”的数学模型，该模型具有2（或更多）修饰位点的蛋白质，这些蛋白质选择性地隔离效应子/辅因子分子可以用作昼夜节律的时间守护者。在现场占用方面，可以使用两个相对简单的非线性ODE实现2个站点机制；这些模型不需要过分特殊的参数来生成稳定的极限周期振荡。
It is possible that there are post-translational circadian oscillators that continue functioning in the absence of negative feedback transcriptional repression in many cell types from diverse organisms. Apart from the KaiABC system from cyanobacteria, the molecular components and interactions required to create in-vitro ("test-tube") circadian oscillations in different cell types are currently unknown. Inspired by the KaiABC system, I provide "proof-of-principle" mathematical models that a protein with 2 (or more) modification sites which selectively sequesters an effector/cofactor molecule can function as a circadian time-keeper. The 2-site mechanism can be implemented using two relatively simple coupled non-linear ODEs in terms of site occupancy; the models do not require overly special fine-tuning of parameters for generating stable limit cycle oscillations.
