ISO INTERNATIONAL 9910 STANDARD First edition 1991-12-15 Ansr fatormet Toc Sen Oil of sweet orange --- Determination of the total carotenoids content Huile essentielle d'orange douce --- Determination de la teneur en carotenoides totaux Reference number ISO 9910:1991(E) st andar d999 ISO 9910:1991(E) Foreword Iso (the International Organization for Standardization) is a worldwide federation of national standards bodies (iso member bodies). The work of preparing International Standards is normally carried out through IsO technical committees. Each member body interested in a subject for which a technical committee has been established has the right to be represented on that committee. International organizations, govern- mental and non-governmentat, in liaison with Iso, also take part in the work. IsO collaborates closely with the International Electrotechnical Commission (IEC) on all matters of electrotechnical standardization. Draft International Standards adopted by the technical committees are circulated to the member bodies for voting. Publication as an Inter- national Standard requires approval by at least 75 % of the member bodies casting a vote. International Standard Iso 9910 was prepared by Technical Committee ISO/TC 54, Essential oils. CISO1991 or by any means, electronic or mechanical, including photocopying and microfilm, without permission in writing from the publisher. International Organization for Standardization Case Postale 56· CH-1211 Geneve 20· Switzerland Printed in Switzerland .ii st andar d999 ISO 9910:1991(E) INTERNATIONAL STANDARD Oil of sweet orange -- Determination of the total carotenoids content 5.2 Beaker, of capacity 50 ml. 1 Scope This International Standard specifies a method for 5.3 One-mark volumetric flask, of capacity 100 ml, the determination of the total carotenoids content of complying with the requirements of iso 1042. oil of sweet orange. 5.4 One-mark pipettes, of appropriate capacity, Normative references complying with the requirements of iso 648. 2 The following standards contain provisions which, 5.5 Analytical balance. through reference in this text, constitute provisions of this International Standard. At the time of publi- 5.6 Cells, of depth 1 cm. cation, the editions indicated were valid. All stan- dards are subject to revision, and parties to agreements based on this Internationai Standard 6 Procedure are encouraged to investigate the possibitity of ap- plying the most recent editions of the standards in- dicated below. Members of IEC and Iso maintain 6.1 Test portion registers of currently valid International Standards. Weigh, to the nearest 1 mg, about 0,5 g of the test iso 648:1977, Laboratory glassware One-mark sample, in a 50 mi beaker (5.2). pipettes. 6.2 Preparation of the test solution Iso 1042:1983, Laboratory glassware --- One-mark volumetric flasks. Dissoive the test portion (6.1) in the solvent (4.1), in a 100 ml one-mark volumetric ffask (5.3), dilute to Principle the mark with solvent and mix. Spectrophotometric measurement of a light pet- 6.3 Determination roleum solution of the oil at maximum absorbance, between 400 nm and 500 nm. Measure the absorbance of the solution between 400 nm and 500 nm and record the maximum Reagents 4. absorbance of the curve (see figure 1), using light petroleum as a blank. 4.1 Solvent, light petroleum, boiling range 40 °c to 60 °C. Expression of results 7 5 Apparatus The total carotenoids content wc, expressed in milligrams per kilogram, is given by the formula Ordinary laboratory apparatus, and d × 0,4 Wc × 1 000 m 5.1 Spectrophotometer, suitable for carrying out where measurements between 400 nm and 500 nm. 1 st andar d99s