ISO INTERNATIONAL STANDARD 18415 Second edition 2017-06 Cosmetics Microbiology Detection of specified and non- specified microorganisms Cosmetiques - Microbiologie - Deétection des micro-organismes spécifiés et non spécifiés Reference number IS0 18415:2017(E) International Organization for Standardization @ IS0 2017 =ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under lic Not for Resale, 2017/8/21 02:11:49 itted without license from IHS IS0 18415:2017(E) COPYRIGHTPROTECTEDDOCUMENT IS02017,PublishedinSwitzerland All rights reserved. Unless otherwise specified, no part of this publication may be reproduced or utilized otherwise in any form or by any means, electronic or mechanical, including photocopying, or posting on the internet or an intranet, without prior written permission. Permission can be requested from either ISO at the address below or ISO's member body in the country of the requester. ISO copyright office Ch. de Blandonnet 8 . CP 401 CH-1214 Vernier, Geneva, Switzerland Tel.+41 22 749 01 11 Fax +41227490947 [email protected] www.iso.org Intenationai br PrganizationforStandardization icensee-ZHEJIANG INST OF STANDARDIZRoISQ s1.7 - All rights reserved I by IHS unde Not for Resale, 2017/8/21 02:11:49 etworking permitted without license from IHS IS0 18415:2017(E) Contents Page Foreword ..V Introduction. ...vi 1 Scope. 2 Normative references 3 Terms and definitions 4 Principle ..3 5 Diluents and culture media .3 5.1 General .3 5.2 Diluent for the microbial suspension (tryptone sodium chloride solution) .3 5.2.1 General .3 5.2.2 Composition ..4 5.2.3 Preparation. .4 5.3 Culture media. .4 5.3.1 General. 4 5.3.2 Enrichment broth. ..4 5.3.3 Non-selective agar medium ..5 6 5 Apparatus and glassware 7 Strains of microorganism. ..6 8 Handling of cosmetic products and laboratory samples ..6 9 Procedure ..6 9.1 General recommendations. 9.2 Preparation of the initial suspension in the enrichment broth .6 9.2.1 General. ..6 9.2.2 Water-miscible products .7 9.2.3 Water-immiscible products 9.2.4 Filterable products. 7 9.3 Incubation of the initial suspension .7 9.4 Isolation of specified and non-specified microorganisms. 7 9.5 Procedure for identification of the specified microorganism: Pseudomonas aeruginosa. 9.5.1 Gram staining. 7 9.5.2 Oxidasetest 7 9.5.3 Identification test .8 9.6 Procedure for identification of the specified microorganism: Escherichia coli 8 9.6.1 Gram staining. 8 9.6.2 Oxidasetest .8 9.6.3 Identification test .8 9.7 Procedure for identification of the specified microorganism: Staphylococcus aureus .8 9.7.1 Gram staining. 8 9.7.2 Catalasetest .8 9.7.3 Identification test .8 9.8 Procedure for the identification of the specified microorganism: Candida albicans .9 9.8.1 Gram staining. 9 9.8.2 Identification test .9 9.9 Procedure for the identification of non-specified microorganisms .9 9.9.1 Gram staining .9 9.9.2 Oxidasetest .9 9.9.3 Catalase test .9 9.9.4 Identification test. .9 10 Expression of the results .10 10.1 Detection of specified microorganisms .10 iii nSee=ZHEJIANG INST OF STANDARDIZATION C1 5956617 vided by IHS under license with ISC Not for Resale, 2017/8/21 02:11:49 ithout license from IHS